Journal: Stem Cell Research & Therapy

Article Title: CXCL14 and MCP1 are potent trophic factors associated with cell migration and angiogenesis leading to higher regenerative potential of dental pulp side population cells

doi: 10.1186/s13287-015-0088-z

Figure Lengend Snippet: Characterization of regenerated tissue on day 28 in an ectopic tooth root transplantation model. a , f , k , o , t Transplant of pulp CD31 − side population (SP) cells (Pulp SCs). b , g , l , p , u Transplant of conditioned medium (CM) from pulp CD31 − SP cells (Pulp CM). c , h , m , q , v Transplant of CM from bone marrow CD31 − SP cells (BM CM). d , i , n , r , w Transplant of CM from adipose CD31 − SP cells (AD CM). a - d Immunostaining with rat endothelial cell antigen 1 (RECA1). e Ratio of vascularization area to the total regenerated area. ( f - i ) In situ hybridization analysis of expression of thyrotropin-releasing hormone-degrading enzyme ( TRH-DE ) as a pulp marker using an anti-sense probe reactive to both porcine and mouse genes. j Protein expression of TRH-DE in regenerated pulp after transplantation of CM from pulp, bone marrow (BM), and adipose (AD) CD31 − SP cells. k - s Odontoblastic differentiation potential in the regenerated pulp. k - n Odontoblastic cells along with the dentinal wall. o - r In situ hybridization analysis of enamelysin . Odontoblastic process extending into the tubular dentin (arrows). s Comparison of the numbers of enamelysin -positive cells along the dentinal wall. t - w Immunostaining with aggrecan (green) merged with Hoechst 33342 (Blue). x Ratio of aggrecan-positive area to the total regenerated area. Data are expressed as mean ± standard deviation of four determinations. * P < 0.05, ** P < 0.01

Article Snippet: Immunohistological analyses with mouse anti-rat RECA1 (rat endothelial cell antigen 1) (Sanbio BV, Uden, The Netherlands) (1:500) with biotinylated horse anti-mouse Texas Red secondary antibody (Vector Laboratories, Burlingame, CA, USA) (1:200) were performed to determine the level of neovascularization.

Techniques: Transplantation Assay, Immunostaining, In Situ Hybridization, Expressing, Marker, Standard Deviation

Journal: Stem Cell Research & Therapy

Article Title: CXCL14 and MCP1 are potent trophic factors associated with cell migration and angiogenesis leading to higher regenerative potential of dental pulp side population cells

doi: 10.1186/s13287-015-0088-z

Figure Lengend Snippet: Localization of monocyte chemotactic protein 1 (MCP1) and its receptor, CCR2, related to the transplanted cells and the endogenous migrated cells in the regenerated pulp. a , b Immunostaining of MCP1 (red) merged with glutamic-oxaloacetic transaminase 2 (GOT2) or bromodeoxyuridine (BrdU) (green) and Hoechst 33342 (blue) 7 days after transplantation of pulp CD31 − side population (SP) cells. c - e In situ hybridization and immunohistochemical analysis of expression of CCR2 (red) merged with BrdU (green) 7 days after transplantation of conditioned media (CM) from pulp ( c ), bone marrow ( d ), and adipose CD31 − SP cells ( e ). f Ratio of CCR2-positive cell numbers to BrdU-positive cell numbers in the regenerated pulp. Data are expressed as mean ± standard deviation of four determinations. * P < 0.05. g - j MCP1-positive cells (green) in proximity to the newly formed vessels immunostained with rat endothelial cell antigen 1 (RECA1) (red) 28 days after transplantation of pulp CD31 − SP cells ( g ), CM of pulp ( h ), CM of bone marrow ( i ), and CM of adipose CD31 − SP cells ( j ). Note no co-localization. k The number of MCP1-positive cells related to the diameter of blood vessels

Article Snippet: Immunohistological analyses with mouse anti-rat RECA1 (rat endothelial cell antigen 1) (Sanbio BV, Uden, The Netherlands) (1:500) with biotinylated horse anti-mouse Texas Red secondary antibody (Vector Laboratories, Burlingame, CA, USA) (1:200) were performed to determine the level of neovascularization.

Techniques: Immunostaining, Transplantation Assay, In Situ Hybridization, Immunohistochemical staining, Expressing, Standard Deviation